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Transfection of HBL cells was performed by liposome-mediated gene transfer in 6 or well plates. Infectious Diseases, University of Basel, Switzerland. Normalized data were subsequently presented as relative quantification. Structure and function of simian virus 40 large tumor antigen. Discussion Polyomavirus large tumor-antigen LTag interacts with a number of host molecules involved with cell cycle, including the tumor-suppressor gene product p53 and has been identified as an important target of cancer immunity in murine models[ 38 ].
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This article has been cited by other articles in PMC. In humans, however, despite continuous efforts aimed at identifying potential HLA class I restricted LTag epitopes[ 40 , 41 ], an epitope-specific cytotoxic immune response against this antigens or its portions is still poorly characterized [ 42 , 43 ]. T-cell responses to peptide fragments of the BK virus T antigen: The fusion proteins and GST were coated on microtiter plates according to standard procedures. National Center for Biotechnology Information , U.
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A standardization of peptides' scores was applied to identify best binders according to their final values using the following formula: A threshold of 2-fold increase over the baseline was considered as a cut-off. Acknowledgements The authors thank Dr. Oncogenic transformation by BK virus and association with human tumors. Prospective study of polyomavirus type BK replication and nephropathy in renal-transplant recipients. Recent investigations have associated them with the outgrowth of specific cancer types including colorectal cancer[ 10 , 11 ], glioblastomas[ 12 , 13 ], mesotheliomas[ 14 ], prostate cancer[ 15 ] and, possibly, lymphomas[ 16 , 17 ].
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Cytotoxic activity was then tested against specific squares or control diamonds peptide-loaded targets. Published online Nov Received Oct 20; Accepted Nov HLA-class I restricted immune responses against defined viral antigens can be frequently induced by ex vivo stimulation of peptide-specific T cells from seropositive subjects[ 45 ]. Support Center Support Center. Specific cytotoxicity values at The role of human polyomaviruses in human cancer is still debated.
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